BelTox, together with the European Society of Toxicology In Vitro (ESTIV), organized the “Applied In Vitro Toxicology” course from 8 to 13 January 2017 in Belvaux-Luxembourg. The course was hosted by the Luxembourg Institute for Science and Technology (LIST) and was attended by 24 participants. Most course attendees had an industrial background and came from 11 different countries, including Belgium, the Czech Republic, Denmark, Estonia, France, Germany, Poland, Portugal, Romania, Switzerland and the USA. The program consisted of 17 lectures in the area of regulatory, investigative and screening in vitro toxicology. During the group exercise, the course attendees applied all theoretically acquired in vitro skills to real-life cases of toxicologically relevant chemicals. Furthermore, the participants were given the opportunity to gain hands-on experience, in particular regarding tridimensional in vitro cell culture modelling and in vitro skin irritation testing, the latter being organized by the course sponsor CellSystems. Based on a survey filled out by the participants, the course was rated of high quality.